Analysis of human neutrophils from nasal polyps by single-cell RNA sequencing reveals roles of neutrophils in chronic rhinosinusitis.

ABSTRACT:

Background

Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by type 2 (T2) inflammation. Recent studies, including our own, suggest that neutrophils are also elevated in T2 nasal polyps (NP) and that elevated neutrophils display an activated phenotype. However, the actual roles of neutrophils in NP pathogenesis in T2 CRSwNP are still largely unclear.

Objective

To reveal the roles and heterogeneity of neutrophils in NP tissue by single-cell RNA sequencing analysis.

Methods

We developed a novel microwell-based single-cell RNA sequencing assay using granulocyte-enriched samples from 5 control sinus tissues, 5 NP tissues and patient-matched peripheral blood (PB) samples. This approach allowed for examination of differential expression of genes in NP neut

SUBMITTER: Iwasaki N 

PROVIDER: S-EPMC11875937 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature