Importance of mast cell histamine secretion in IgG-mediated systemic anaphylaxis.

ABSTRACT:

Background

IgG can mediate murine and human systemic anaphylaxis (SA). The roles of mast cells (MCs) and histamine in IgG-mediated anaphylaxis are controversial for mice and have not been studied in vivo for humans. We are now investigating these issues.

Methods

Actively or passively sensitized wild-type and immune-deficient mice were induced to develop anaphylaxis by intravenous antigen challenge. Anaphylaxis was characterized by evaluating hypothermia, hypomobility, histamine, and MC protease responses.

Results

In contrast to our previous results with protein-immunized mice from a conventional colony, IgG-mediated passive SA in our specific pathogen-free colony mice depended considerably on histamine produced by connective tissue MCs (CTMCs) in response to FcγRIII c

SUBMITTER: Khodoun MV 

PROVIDER: S-EPMC11875995 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature