Spectroscopic characterization of bacterial colonies through UV hyperspectral imaging techniques.

ABSTRACT:

Introduction

Plate culturing and visual inspection are the gold standard methods for bacterial identification. Despite the growing attention on molecular biology techniques, colony identification using agar plates remains manual, interpretative, and heavily reliant on human experience, making it prone to errors. Advanced imaging techniques, like hyperspectral imaging, offer potential alternatives. However, the use of hyperspectral imaging in the VIS-NIR region has been hindered by sensitivity to various components and culture medium changes, leading to inaccurate results. The application of hyperspectral imaging in the ultraviolet (UV) region has not been explored, despite the presence of specific absorption and emission peaks in bacterial components.

Methods

To address this

SUBMITTER: Ezenarro JJ 

PROVIDER: S-EPMC11876133 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature