Transcriptomic and proteomic analysis of oil body associated protein dynamics in the biofuel feedstock Pennycress (<i>Thlaspi arvense</i>).

ABSTRACT: Pennycress (Thlaspi arvense) is an emerging feedstock for biofuel production because of its high seed oil content enriched in erucic acid. A combination of transcriptomic and proteomic tools was used to characterize the dynamics and relative abundance of the major oil body related proteins in the Pennycress seed. Our analysis identified 21 oleosins (OLE), 6 oil body associated proteins (OBAPs), 3 SEIPINS, 3 caleosins, 3 stereolisins and 3 lipid droplet associated proteins (LDAPs) in the Pennycress genome, showing high homology with respect to Arabidopsis or rapeseed. RNA-Seq analysis on five Pennycress seed maturation stages showed that most OLE and OBAP genes increased their expression with seed maturation, coinciding with the highest accumulation of triacylglycerol.

SUBMITTER: Lujan MA 

PROVIDER: S-EPMC11876164 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature