Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction.

ABSTRACT: Leveraging a depth profiling approach expands the chemical elucidation of mass spectrometry imaging techniques to another dimension. Three-dimensional MSI (3D MSI) reveals the distribution of analytes with greater anatomical detail to add another level of information in a biological study. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) has demonstrated utility for an ablation-based approach, enabling simplified sample preparation workflows and streamlined data processing pipelines compared to a serial-sectioning strategy. To improve 3D MSI on the IR-MALDESI platform, two technologies have been characterized in tandem for the intention of minimizing sampling bias: (1) a top-hat optical train and (2) a chromatic confocal probe (CA probe). While the modified op

SUBMITTER: Sohn AL 

PROVIDER: S-EPMC11876208 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature