Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts.

ABSTRACT:

Purpose

Acanthamoeba castellanii is an important opportunistic human protozoal pathogen that can cause both skin, ocular and brain infections. Recent studies have established that brews and solvent extract (SE) of green tea (Camellia sinensis) can inhibit the growth and encystation of A. castellanii. Here we characterized those growth and encystation inhibitions.

Methods

Herein, we characterize of the morphological and chemical changes that occur in the trophozoites and the encysting stage of A. castellanii after exposure to C. sinensis SE and brew using Transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) microspectroscopy and fluorescence-based assays.

Results

TEM showed ultrastructural changes in both A. castellanii stages. FTIR microspectrosco

SUBMITTER: Fakae LB 

PROVIDER: S-EPMC11876251 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature