Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4.

ABSTRACT: The non-structural protein 4 (NSP4) of porcine reproductive and respiratory syndrome virus (PRRSV) is equipped with 3C-like serine protease (3CLSP) activity, influencing crucial aspects such as virus replication, host IFN-β suppression, host cell apoptosis induction, and PRRSV detection facilitation. In response to wild or attenuated PRRSV strains, antibodies against non-structural proteins are generated, while inactivated vaccines fail to elicit such responses. Employing the Enzyme-Linked Immunosorbent Assay (ELISA) method targeting non-structural proteins helps discern the immune effects of inactivated versus wild or attenuated vaccine strains. The study focused on the NSP4 protein from the PRRSV XH-GD strain (GenBank No. EU624117.1), which was cloned, expressed, and leveraged as

SUBMITTER: Zhao M 

PROVIDER: S-EPMC11876416 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature