Designing and Evaluation of a Plasmid Encoding Immunogenic Epitopes From <i>Echinococcus granulosus</i> Eg95-1-6, P29, and GST Against Hydatid Cyst in BALB/c Mice.

ABSTRACT: Cystic echinococcosis (CE) is a neglected parasitic infection with a particular impact in humans and livestock. The current investigation was undertaken to design and evaluate a DNA vaccine encoding Echinococcus granulosus Eg95-1 to EG95-6, P29, and GST against hydatid cyst infection in BALB/c mice. Initially, B-cell, cytotoxic T-lymphocyte, and helper T-lymphocyte epitopes were forecasted using B-cell epitope prediction server (BCPREDS) and Immune Epitope Database (IEDB) server, respectively, and a vaccine construct incorporating multiple epitopes was rationally designed and comprehensively analyzed through in silico modeling and simulation studies. Next, Escherichia coli TOP10 was transformed by the recombinant pcDNA 3.1 plasmid and mass production, followed by plasmid extr

SUBMITTER: Khazaei S 

PROVIDER: S-EPMC11876539 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature