The difference between MelP5 and melittin membrane poration.

ABSTRACT: Melittin, a natural peptide found in bees, has been shown to induce pore formation in cell membranes. However, its artificial mutant, MelP5 can do so at concentrations 200 times lower than melittin. The mechanism of the enhanced portion ability is not fully understood. By conducting all-atom molecular dynamics (MD) simulations, we found that MelP5 forms a stable pore that is macro-molecular sized. Our results suggest that the mutation of five amino acids from melittin reduces the electrostatic repulsion between peptides and strengthens hydrophobic interactions between MelP5 and lipid tails, resulting in the formation of a stable and larger pore. Furthermore, we found that cholesterol (CHOL), which occupies 30% in mammalian cell membranes, plays a crucial role in enhancing the pore formatio

SUBMITTER: Zan B 

PROVIDER: S-EPMC11876596 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature