Metabolic mutations reduce antibiotic susceptibility of E. coli by pathway-specific bottlenecks.

ABSTRACT: Metabolic variation across pathogenic bacterial strains can impact their susceptibility to antibiotics and promote the evolution of antimicrobial resistance (AMR). However, little is known about how metabolic mutations influence metabolism and which pathways contribute to antibiotic susceptibility. Here, we measured the antibiotic susceptibility of 15,120 Escherichia coli mutants, each with a single amino acid change in one of 346 essential proteins. Across all mutants, we observed modest increases of the minimal inhibitory concentration (twofold to tenfold) without any cases of major resistance. Most mutants that showed reduced susceptibility to either of the two tested antibiotics carried mutations in metabolic genes. The effect of metabolic mutations on antibiotic susceptibility was ant

SUBMITTER: Lubrano P 

PROVIDER: S-EPMC11876631 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature