Intraneoplastic fungal dysbiosis is associated with colorectal cancer progression and host gene mutation.

ABSTRACT:

Background

The relationship between intraneoplastic fungi and colorectal cancer (CRC) progression remains largely unclear. Here, we investigated fungal community changes in adenoma and CRC and their correlation with host genetic mutations.

Methods

We obtained 261 tissue biopsies from two geographically distinct cohorts of CRC and adenoma patients, with each individual contributing 2-5 biopsies from lesions and 2 from adjacent normal tissues. 18S ribosomal RNA gene sequencing was used for microbial profiling. Host genetic alterations including KRAS mutations and microsatellite instability (MSI) were detected concurrently.

Findings

Intra-neoplastic fungal composition significantly differed between CRC and adenoma in two independent cohorts, with enrichment of highly var

SUBMITTER: Yuan K 

PROVIDER: S-EPMC11876754 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature