Colorimetric CRISPR Biosensor: A Case Study with Salmonella Typhi.

ABSTRACT: There is a critical need to implement a sensitive and specific point-of-care (POC) biosensor that addresses the instrument limitations and manufacturing challenges faced in resource-constrained contexts. In this paper we focus on enteric fever which is a highly contagious and prevalent infection in low- and middle-income countries. Although easily treatable, its ambiguous symptoms paired with a lack of fast, accurate and affordable diagnostics lead to incorrect treatments which exacerbate the disease burden, including increasing antibiotic resistance. In this study, we develop a readout module for CRISPR-Cas12a that produces a colorimetric output that is visible to the naked eye and can act as a cascade signal amplifier in any CRISPR assay based on trans-cleavage. We achieve this by immobi

SUBMITTER: Pascual-Garrigos A 

PROVIDER: S-EPMC11877495 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature