Electrode- and Label-Free Assessment of Electrophysiological Firing Rates through Cytochrome C Monitoring via Raman Spectroscopy.

ABSTRACT: In vitro neurotoxicology aims to assess and predict the side effects of exogenous chemicals toward the human brain. Among the exploited approaches, electrophysiological techniques stand out for the high spatiotemporal resolution and sensitivity, with the patch clamp considered the gold standard technique for such purposes. However, structural toxicity and metabolic effects may elude detection when only the electrical activity is measured, highlighting the need for integrating electrophysiological recordings with complementary approaches such as optical methods. In this study, we describe an integrated platform for recording neuronal electrical activity and performing chemical analysis with a noninvasive label-free optical imaging, Raman spectroscopy. Specifically, we developed a pro

SUBMITTER: Tentellino C 

PROVIDER: S-EPMC11877498 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature