Single Nucleotide Polymorphism Highlighted via Heterogeneous Light-Induced Dissipative Structure.

ABSTRACT: The unique characteristics of biological structures depend on the behavior of DNA sequences confined in a microscale cell under environmental fluctuations and dissipation. Here, we report a prominent difference in fluorescence from dye-modified single-stranded DNA in a light-induced assembly of DNA-functionalized heterogeneous probe particles in a microwell of several microliters in volume. Strong optical forces from the Mie scattering of microparticles accelerated hybridization, and the photothermal effect from the localized surface plasmons in gold nanoparticles enhanced specificity to reduce the fluorescence intensity of dye-modified DNA to a few %, even in a one-base mismatched sequence, enabling us to clearly highlight the single nucleotide polymorphisms in DNA. Fluorescence intensity

SUBMITTER: Toyouchi S 

PROVIDER: S-EPMC11877512 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature