NONO regulates m⁵C modification and alternative splicing of PTEN mRNAs to drive gastric cancer progression.

ABSTRACT:

Background

The effect of m⁵C modification on oncogene mRNAs has been well studied, while little is known about its influence on mRNAs of tumor suppressor genes (TSGs). Early studies showed PTEN, a key TSG, undergoes alternative splicing (AS) in cancers, however, the underlying regulatory mechanism remains elusive.

Methods

We analyzed tissue microarrays and transcriptomic data derived from gastric cancer, with an emphasis on RNA splicing and m⁵C regulators. To unravel the role of NONO in GC, we employed RNA sequencing, RNA-Bis-Seq, RNA immunoprecipitation, RNA in situ hybridization, and Minigene reporter assay with NONO knockdown cells. The clinical relevance was validated using CDX models and human tissue microarrays.

Results

Analysis of publicly

SUBMITTER: Zhao G 

PROVIDER: S-EPMC11877715 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature