Functional validation to explore the protective role of miR-223 in Staphylococcus aureus-induced bovine mastitis.

ABSTRACT:

Background

Mastitis caused by Staphylococcus aureus (S. aureus) is one of the most intractable problems for the dairy industry, causing significantly reduced milk yields and early slaughter of cows worldwide. MicroRNAs (miRNAs) can post-transcriptionally regulate gene expression and studies in recent years have shown the importance of miRNA-associated gene regulation in S. aureus-induced mastitis.

Results

In this study, to investigate the role of miR-223 in mastitis, we performed experiments to overexpress and suppress miR-223 in an immortalized bovine mammary epithelial cell line (MAC-T) infected with S. aureus. Overexpression of miR-223 in MAC-T cells repressed cell apoptosis and necrosis induced by S. aureus infection, whereas suppression of miR-223 had the opposite effec

SUBMITTER: Liu X 

PROVIDER: S-EPMC11877765 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature