Decoding dengue's neurological assault: insights from single-cell CNS analysis in an immunocompromised mouse model.

ABSTRACT:

Background

Dengue encephalitis, a severe neurological complication of dengue virus infection, is increasingly recognized for its rising incidence and significant public health burden. Despite its growing prevalence, the underlying mechanisms and effective therapeutic strategies remain poorly understood.

Methods

Cellular atlas of dengue encephalitis was determined by single-nucleus RNA sequencing. Viral load of dengue virus and the level of cytokines expression was detected by RT-qPCR. The target cells of dengue virus were verified by immunofluorescence. The cytotoxic effect of CD8⁺ T cell was determined by flow cytometry, immunofluorescence, in vivo CD8⁺ T cell depletion, adoptive transfer and CCK-8-based cell viability assay. Axonal and synaptic reduct

SUBMITTER: Qiu M 

PROVIDER: S-EPMC11877810 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature