Impact of fasting and refeeding on immune markers, hepatic gene expression, and gut microbiota in geese: insights into metabolic regulation and gut-liver interactions.

ABSTRACT: Fasting and refeeding protocols, which induce short-term fluctuations in nutrient and energy levels, elicit adaptive physiological responses in animals. In this study, biochemical, transcriptome and 16S rRNA sequencing techniques were used to investigate the physiological effects of fasting and refeeding on immune responses, liver gene expression, and gut microbiota composition in geese. Fasting led to a significant reduction in circulating levels of IgA and IFN-γ, while IgG, TNF-α, IL-6, and IL-10 levels remained stable. Upon refeeding, IgA and IFN-γlevels rapidly returned to baseline. RNA-Seq analysis identified 858 differentially expressed genes (DEGs) between the control and fasted groups, and 732 DEGs between the fasted and refed groups. Key regulatory genes involved in

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11877903 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature