Modulation of mechanosensitive genes during embryonic aortic arch development.

ABSTRACT:

Background

Early embryonic aortic arches (AA) are a dynamic vascular structures that are in the process of shaping into the great arteries of cardiovascular system. Previously, a time-lapsed mechanosensitive gene expression map was established for AA subject to altered mechanical loads in the avian embryo. To validate this map, we investigated effects on vascular microstructure and material properties following the perturbation of key genes using an in-house microvascular gene knockdown system.

Results

All siRNA vectors show a decrease in the expression intensity of desired genes with no significant differences between vectors. In TGFβ3 knockdowns, we found a reduction in expression intensities of TGFβ3 (≤76%) and its downstream targets such as ELN (≤99.6%), Fbn1 (≤60%), COL

SUBMITTER: Siddiqui HB 

PROVIDER: S-EPMC11877992 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature