Post-transcriptional regulation of aromatic amino acid metabolism by GcvB small RNA in <i>Escherichia coli</i>.

ABSTRACT: Escherichia coli synthesizes aromatic amino acids (AAAs) through the common pathway to produce the precursor, chorismate, and the three terminal pathways to convert chorismate into Phe, Tyr, and Trp. E. coli also imports exogenous AAAs through five transporters. GcvB small RNA post-transcriptionally regulates more than 50 genes involved in amino acid uptake and biosynthesis in E. coli, but the full extent of GcvB regulon is still underestimated. This study examined all genes involved in AAA biosynthesis and transport using translation reporter assay and qRT-PCR analysis. In addition to previously verified targets, aroC, aroP, and trpE, we identified new target genes that were significantly repressed by GcvB primarily via the R1 seed region. Excepti

SUBMITTER: Kanda T 

PROVIDER: S-EPMC11878033 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature