<i>In vitro</i> investigations on the impact of fermented dairy constituents on fecal microbiota composition and fermentation activity.

ABSTRACT: Fermented dairy constitutes a major dietary source and contains lactose as the main carbohydrate and living starter cultures, which can encounter the intestinal microbiota after ingestion. To investigate whether dairy-related nutritional and microbial modulation impacted intestinal microbiota composition and activity, we employed static fecal microbiota fermentations and a dairy model system consisting of lactose and Streptococcus thermophilus wild type and β-galactosidase deletion mutant. In addition, we conducted single-culture validation studies. 16S rRNA gene-based microbial community analysis showed that lactose increased the abundance of Bifidobacteriaceae and Anaerobutyricum and Faecalibacterium spp. The supplied lactose was hydrolyzed within 24 h of ferm

SUBMITTER: Li Q 

PROVIDER: S-EPMC11878039 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature