Surveillance for <i>Chlamydia trachomatis</i> variants escaping detection with the Aptima Combo 2 assay in Canada from 2019 to 2021.

ABSTRACT: Nucleic acid amplification tests (NAATs) are the method of choice for Chlamydia trachomatis diagnosis, but these strategies are susceptible to target site mutations. C. trachomatis variants escaping detection with the Aptima Combo 2 (AC2) assay on the Hologic Panther instrument from 23S rRNA mutations have been reported in Nordic countries, England, Japan, and the United States. Given the potential for false negative results, this study investigated whether strains of C. trachomatis with AC2 target site mutations were present in Canada. Surveillance was conducted in Canadian laboratories from 2019 to 2021. Specimens suspected of AC2 target site mutations included those with low-value detections on the AC2 assay, with subsequent high-value detections on the Aptima Chlam

SUBMITTER: Roy F 

PROVIDER: S-EPMC11878061 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature