Determination of <i>Pseudomonas aeruginosa</i> MexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes.

ABSTRACT: Defining the substrates of resistance-nodulation-division systems is often complicated by the presence of multiple systems in a single bacterial species. Using a major efflux knockout strain, we developed a Pseudomonas aeruginosa system that enables controlled expression of MexXY-OprM in the absence of background efflux complexes with overlapping substrate profiles. This system identified three groups of potential substrates: substrates, partial substrates, and nonsubstrates, and defined trimethoprim as a new substrate for MexXY-OprM.IMPORTANCEIncreasing antibiotic resistance in pathogenic bacteria is a major public health concern. One way bacteria can sustain growth in the presence of antibiotics is through active removal of these molecules via efflux pumps. In Pseudomonas aerug

SUBMITTER: Kavanaugh LG 

PROVIDER: S-EPMC11878066 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature