Dissemination of <i>bla</i>_NDM-harboring plasmids in carbapenem-resistant and hypervirulent <i>Klebsiella pneumoniae</i>.

ABSTRACT: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a global public health threat, and CRKP strains carrying bla_NDM plasmids are of particular concern. Here, the antimicrobial resistance and molecular epidemiological characteristics of 59 CRKP strains isolated from patients receiving care at a tertiary hospital in China from 2019 to 2021 were determined through antimicrobial resistance testing and genomic sequence analysis. We analyzed the genetic characteristics and transmissibility of bla_NDM-harboring plasmids through S1-digested pulsed field gel electrophoresis, Southern blotting, and conjugation assays. The virulence levels of the strains were evaluated using a Galleria mellonella larvae infection model. All the CRKP strains were multi

SUBMITTER: Gu Y 

PROVIDER: S-EPMC11878072 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature