Holistic Investigation of Graphene Quantum Dot Endocytosis.

ABSTRACT: Graphene quantum dots (GQDs) have gained popularity in nano-biotechnology due to their multifunctional delivery and imaging capabilities. The outcome of their therapeutic delivery applications relies on understanding cell internalization routes. Current literature presents often conflicting results based on surveying only a few endocytosis inhibitors. Herein, a holistic approach to cell uptake studies by utilizing six different inhibitors while considering their on- and off-target effects on internalization of the GQDs of different charges is provided. Endocytosis paths are explored by tracking intracellular GQD fluorescence in HeLa or HEK-293 cells. Contrary to the previous assumptions of a singular entry route, findings suggest that GQDs enter the cells through several endocytosis paths

SUBMITTER: Topkiran UC 

PROVIDER: S-EPMC11878264 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature