A new methodology for sub-femtomolar detection of organic molecules through the combination of surface-enhanced Raman spectroscopy and a superhydrophobic fluidic concentrator.

ABSTRACT: A specific device that combines (1) surface-enhanced Raman spectroscopy (SERS) and (2) superhydrophobic surfaces is developed to detect traces of analytes diluted at sub-femtomolar concentration in water solutions. The first step of the analysis consists in the evaporation of a drop of the solution on the device, designed to concentrate all the analytes on a central functionalized small area (80 µm diameter). This analytical zone is covered with Ag nanoparticles dedicated to enhance Raman signals. In a second step, this zone is scanned pixel by pixel to accumulate around 2200 Raman spectra. The third step is an algorithmic analysis of the pile of spectra to identify Raman peaks that are specific to the targeted molecules. We detail an original analysis method that allows (1) to select spec

SUBMITTER: Fabre V 

PROVIDER: S-EPMC11878439 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature