Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination.

ABSTRACT: High-energy electrons induce sample damage and motion at the nanoscale to fundamentally limit the determination of molecular structures by electron diffraction. Using a fast event-based electron counting (EBEC) detector, we characterize beam-induced, dynamic, molecular crystal lattice reorientations (BIRs). These changes are sufficiently large to bring reciprocal lattice points entirely in or out of intersection with the sphere of reflection, occur as early events in the decay of diffracted signal due to radiolytic damage, and coincide with beam-induced migrations of crystal bend contours within the same fluence regime and at the same illuminated location on a crystal. These effects are observed in crystals of biotin, a series of amino acid metal chelates, and a six-residue peptide, sugges

SUBMITTER: Vlahakis N 

PROVIDER: S-EPMC11878443 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature