Runx-dependent expression of PKC is critical for cell survival in the sea urchin embryo.

ABSTRACT:

Background

Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. To discover fundamental Runx functions in the cell biology of animal development, we have employed morpholino antisense-mediated knockdown of the sea urchin Runx protein SpRunt-1. Previously we showed that embryos depleted of SpRunt-1 arrest development at early gastrula stage and underexpress the conventional protein kinase C SpPKC1.

Results

We report here that SpRunt-1 deficiency leads to ectopic cell proliferation and extensive apoptosis. Suppression of the apoptosis by pharmacological inhibition of caspase-3 prevents the ectopic proliferation and rescues gastrulation, indicating t

SUBMITTER: Dickey-Sims C 

PROVIDER: S-EPMC1187879 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature