Evaluation of caffeine modulation of topiramate effect on locomotor activity of zebrafish larvae in pentylenetetrazol-induced seizure model.

ABSTRACT: Epilepsy is a prevalent neurological condition marked by seizures that lead to neurobiological and behavioral impairments. Caffeine (CAF), the world's most consumed stimulant, reportedly affects both epileptic seizures and the efficacy of antiepileptic drugs, particularly topiramate (TPM). This study aimed to investigate the effects of CAF on TPM in a pentylenetetrazol (PTZ)-induced seizure model using zebrafish larvae. Four days post-fertilization Danio rerio larvae were incubated for 18 hours with CAF, TPM, or CAF+TPM, followed by an assessment of locomotor activity. Seizures were induced by adding PTZ to achieve a final concentration of 20 mM. In the PTZ-induced seizure model, the application of CAF in doses over 50 mg/L resulted in a decrease in the average movement. TPM ( > 50 μM) sig

SUBMITTER: Bartoszek A 

PROVIDER: S-EPMC11878912 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature