Extracellular Vesicle-Mediated Secretion of Chlorophyll Biosynthetic Intermediates in the Cyanobacterium Leptolyngbya boryana.

ABSTRACT: Extracellular vesicles (EVs) are derived from outer membranes (OMs) in Gram-negative bacteria and have diverse physiological functions. EV-mediated secretion of monovinyl protochlorophyllide (MV-Pchlide), the chlorophyll a (Chl) biosynthetic intermediate, was previously reported in a mutant lacking dark-operative Pchlide reductase in the cyanobacterium Leptolyngbya boryana. This study showed a detailed characterization of EVs from wild-type (WT) strain of L. boryana grown under photoautotrophic and dark heterotrophic conditions, focusing on the accumulation of Chl intermediates. WT L. boryana cells produce two types of EVs, low-density EVs (L-EVs) and high-density EVs (H-EVs), both under light and dark conditions. L-EVs and H-EVs showed distinct morphological features and protein compositi

SUBMITTER: Usui K 

PROVIDER: S-EPMC11879085 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature