The conserved molecular mechanism of erectile dysfunction in type 2 diabetes rats and mice by cross-species transcriptomic comparisons.

ABSTRACT:

Background

The poor clinical situation of type 2 diabetes-induced erectile dysfunction (T2DMED) creates an urgent need for new therapeutic targets.

Aim

To reveal the conserved molecular mechanism of T2DMED across species.

Methods

T2DMED rat and mouse models were constructed to extract mRNA from corpus cavernosum for high-throughput sequencing. The differentially expressed genes (DEGs) were analyzed and the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Protein-Protein Interaction Networks were performed by bioinformatics methods. Immunohistochemistry, immunofluorescence, hematoxylin- eosin and Masson staining were used for subsequent verification.

Outcomes

Cross-species transcriptomics of T2DMED rats and mice were analyzed and validated

SUBMITTER: Xiao M 

PROVIDER: S-EPMC11879191 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature