Delayed polarization of mononuclear phagocyte transcriptional program by type I interferon isoforms.

ABSTRACT:

Background

Interferon (IFN)-alpha is considered a key modulator of immunopathological processes through a signature-specific activation of mononuclear phagocytes (MPs). This study utilized global transcript analysis to characterize the effects of the entire type I IFN family in comparison to a broad panel of other cytokines on MP previously exposed to Lipopolysaccharide (LPS) stimulation in vitro.

Results

Immature peripheral blood CD14+ MPs were stimulated with LPS and 1 hour later with 42 separate soluble factors including cytokines, chemokines, interleukins, growth factors and IFNs. Gene expression profiling of MPs was analyzed 4 and 9 hours after cytokine stimulation. Four hours after stimulation, the transcriptional analysis of MPs revealed two main classes of cytokines:

SUBMITTER: Stroncek DF 

PROVIDER: S-EPMC1187927 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature