Low-density granulocytes and neutrophil extracellular trap formation are increased in incomplete systemic lupus erythematosus.

ABSTRACT:

Objective

To investigate the proportion of low-density granulocytes (LDGs), circulating plasma neutrophil extracellular traps (NETs) and serum-induced NET formation in patients with incomplete SLE (iSLE) and SLE.

Methods

LDGs were measured cross-sectionally in 18 iSLE patients, 11 SLE patients and 14 healthy controls (HCs), whereas circulating NETs and serum-induced NET formation were assessed in 35 iSLE patients, 41 SLE patients and 16 HCs. LDGs (CD14lowCD15+) were measured in peripheral blood mononuclear cells (PBMCs) using flow cytometry, and circulating plasma NETs were measured using anti-myeloperoxidase-DNA, anti-citrullinated histone H3 and anti-elastase-DNA complex ELISAs. Serum-induced NET formation was assessed by incubating healthy neutrophils with serum from iSLE

SUBMITTER: Henning S 

PROVIDER: S-EPMC11879334 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature