Molecular insights into TT2-type MYB regulators illuminate the complexity of floral flavonoids biosynthesis in <i>Freesia hybrida</i>.

ABSTRACT: Proanthocyanidins (PAs), anthocyanins, and flavonols are key flavonoids that play diverse roles in plant physiology and human health. Despite originating from a shared biosynthetic pathway, the regulatory mechanisms of PA biosynthesis and the cooperative regulation of different kinds of flavonoids remain elusive, particularly in flower tissues or organs. Here, we elucidated the regulatory network governing PA biosynthesis in Freesia hybrida 'Red River®' by characterizing four TT2-type MYB transcription factors, designated FhMYBPAs. Phylogenetic analysis, subcellular localization, and transactivation assays predicted their roles as PA-related activators. Pearson correlation analysis revealed significant correlations between FhMYBPAs and PA accumulation in various floral tissues and d

SUBMITTER: Shan X 

PROVIDER: S-EPMC11879501 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature