The transcription factor WRKY41-FLAVONOID 3'-HYDROXYLASE module fine-tunes flavonoid metabolism and cold tolerance in potato.

ABSTRACT: Cold stress adversely affects crop growth and productivity. Resolving the genetic basis of freezing tolerance is important for crop improvement. Wild potato (Solanum commersonii) exhibits excellent freezing tolerance. However, the genetic factors underlying its freezing tolerance remain poorly understood. Here, we identified flavonoid 3'-hydroxylase (F3'H), a key gene in the flavonoid biosynthesis pathway, as highly expressed in S. commersonii compared with cultivated potato (S. tuberosum L.). Loss of ScF3'H function impaired freezing tolerance in S. commersonii, while ScF3'H overexpression in cultivated potato enhanced its freezing tolerance. Metabolic analysis revealed that F3'H generates more downstream products by adding hydroxyl (-OH) groups to the flavonoid ring structures. These fla

SUBMITTER: Bao H 

PROVIDER: S-EPMC11879589 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature