Expression of UCOE and HSP27 Molecular Elements to Improve the Stable Protein Production on HEK293 Cells.

ABSTRACT: Recombinant proteins represent one of the greatest achievements of modern pharmaceutical biotechnology, as they are increasingly used across nearly all branches of medicine to treat a wide range of conditions. In response to this demand, various cell engineering approaches have been developed to improve their expression. Some of these approaches involve the use of genetic elements that prevent the silencing of the gene of interest, as well as the generation of resistant cell lines to inhibit or avoid programmed cell death (PCD). This research focuses on analyzing the effects of overexpression of UCOE elements and the HSP27 protein, both individually and together, on the production of human rIFNγ in HEK293 cells. Our results show that 4-Kb UCOE elements have no effect on protein prod

SUBMITTER: Sosa-Garcia C 

PROVIDER: S-EPMC11879590 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature