Tau and MAP6 establish labile and stable domains on microtubules.

ABSTRACT: We previously documented that individual microtubules in the axons of cultured juvenile rodent neurons consist of a labile domain and a stable domain and that experimental depletion of tau results in selective shortening and partial stabilization of the labile domain. After first confirming these findings in adult axons, we sought to understand the mechanism that accounts for the formation and maintenance of these microtubule domains. We found that fluorescent tau and MAP6 ectopically expressed in RFL-6 fibroblasts predominantly segregate on different microtubules or different domains on the same microtubule, with the tau-rich ones becoming more labile than in control cells and the MAP6-rich ones being more stable than in control cells. These and other experimental findings, which we studi

SUBMITTER: Kirimtay K 

PROVIDER: S-EPMC11879653 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature