Clinical utility of nanopore-targeted sequencing for diagnosing and treating pulmonary infectious diseases from bronchoalveolar lavage fluid.

ABSTRACT:

Background

Conventional microbial testing (CMTs) for infectious pathogens faces challenges in rapid and comprehensive detection. Nanopore-targeted sequencing (NTS) is a novel approach for rapid identification of pathogens; however, clinical experience with the application of NTS is limited.

Methods

We evaluated the diagnostic value of NTS for detecting microbes in bronchoalveolar lavage fluid samples in patients with pulmonary infectious disease (PID, 137 cases), non-pulmonary infectious disease (NPID, 32 cases), or with an unknown etiology (11 cases). We performed a comparative analysis of the diagnostic efficacy of NTS and CMTs in identifying pulmonary infectious diseases and investigated the clinical utility of NTS as a diagnostic tool.

Results

NTS was significantl

SUBMITTER: Li G 

PROVIDER: S-EPMC11879936 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature