Effects of Th1/Th17 and Th2 cytokines on lipid metabolism in differentiated keratinocytes.

ABSTRACT:

Introduction

Abnormalities of keratinocyte differentiation and impairment of permeability barrier are features of inflammatory skin diseases driven by Th1/Th17 and Th2 immune response, such as psoriasis and atopic dermatitis. We aimed at identifying the signature of the Th1/Th17 and Th2 environments on keratinocytes, focusing on the expression of genes involved in the lipid metabolism and profiles of abundance of lipid metabolites.

Methods

Human immortalized keratinocytes in prodifferentiative conditions induced by increasing calcium concentration, and 3D epidermal equivalents were treated with mixtures either of TNF-α and IL-17A plus Th1-related cytokines (IL-1α, IL-6) or of Th2 cytokines (IL-4, IL-13). The expression of genes involved in epidermal differentiation and lipid

SUBMITTER: Cavallo A 

PROVIDER: S-EPMC11880217 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature