Male mouse skeletal muscle lacking HuR shows enhanced glucose disposal at a young age.

ABSTRACT:

Introduction

Metabolic flexibility is the ability of a system to switch between metabolic substrates. Human and murine skeletal muscle tissues and cells with decreased activity of the regulatory RNA-binding protein, human antigen R (HuR), have decreased capacity for fat oxidation, and thus decreased metabolic flexibility. In this study, we aimed to assess the preference for carbohydrates in mice lacking HuR in skeletal muscle.

Methods

Experiments were performed on weight-matched control and HuR knockout mice of both sexes. Palmitate and pyruvate oxidation were performed in mouse muscle following the release of ¹⁴CO₂. In vivo glucose and lipid uptake were assayed in mouse tissue following nonmetabolizable ³H-2-deoxyglucose or ¹⁴

SUBMITTER: Noland RC 

PROVIDER: S-EPMC11880248 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature