ZNF32 histidine 179 and 183 single-site and double-site mutations promote nuclear speckle formation but differentially regulate the proliferation of breast cancer cells.

ABSTRACT: Studies have shown that histidine 179A and 183A (H^{179, 183}A) of the ZNF32 protein exhibit point-like nuclear speckles, but the causes of such speckle formation and their effects on breast cancer cells remain unknown. In this study, we prepared breast cancer cells containing ZNF32 H^{179, 183}A, H¹⁷⁹A, and H¹⁸³A and observed nuclear speckles in all three cell types. Transcriptome analysis showed that these nuclear speckles may be related to changes in the activities of the cell growth factor and RNA polymerase II transcription factor. Comprehensive transcriptomics and metabolomics analyses showed that the formation of ZNF32 nuclear speckles was accompanied by changes in choline metabolism. Both in vivo and in vitro experiments suggested

SUBMITTER: Zhong C 

PROVIDER: S-EPMC11880268 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature