Potential asthma biomarkers identified by nontargeted proteomics of extracellular vesicles in exhaled breath condensate.

ABSTRACT:

Background

Bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) are often misdiagnosed or undiagnosed, highlighting the need for more noninvasive and accessible diagnostic tools. Although exhaled breath condensate (EBC) is recognized as a biomarker resource for respiratory diseases, nontargeted proteomics of extracellular vesicles (EVs) in EBC has not been explored.

Objective

Our aim was to identify protein signatures in EBC-derived EVs (EBC-EVs) and potential biomarkers for BA and COPD.

Methods

EBC-EVs were isolated from 8 patients with BA, 5 patients with COPD, and 9 healthy controls by using the phosphatidylserine affinity method. The isolated EBC-EVs were analyzed by using data-independent acquisition proteomics to identify differentially express

SUBMITTER: Hara R 

PROVIDER: S-EPMC11880581 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature