Inhibitory effects of AptaminC320 targeting vitamin C on LPS-induced inflammation in RAW264.7 cells.

ABSTRACT: Inflammation, a vital immune response, is regulated by macrophages. Key regulators of this response in macrophages are the nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) pathways. This study explored the anti-inflammatory effects of vitamin C and AptaminC320 in macrophages. We found that nitric oxide, produced by lipopolysaccharide (LPS), was reduced by vitamin C and AptaminC320 in RAW264.7 cells, which are murine macrophages. Furthermore, these substances reduced the production of inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6, and interleukin-1β. We also demonstrated that protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), increased by LPS in macrophages, as well as the phosphorylation of c-

SUBMITTER: Choi S 

PROVIDER: S-EPMC11880714 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature