Iterative selection of lipid nanoparticle vaccine adjuvants for rapid elicitation of tumoricidal CD8⁺ T cells.

ABSTRACT: A challenge for cancer vaccines is to elicit immune responses of sufficient magnitude to control malignant tumor growth and spread. In this study, we iteratively screened a panel of 22 lipid-phase vaccine adjuvants in mice for the elicitation of neoantigen-specific CD8⁺ T cell responses, using an integrated peptide-lipid nanoparticle approach. CL401, a dual Toll-like receptor 2/7 (TLR2/7) adjuvant rapidly induced neoantigen-specific T cell responses and improved lymphatic drainage and uptake of the particle. Additional rounds of in vivo screening identified complementary adjuvants which targeted TLR4 (3D6A-PHAD adjuvant), TLR8 (motolimod), and inflammasome (QS-21) pathways and synergized to enhance cytokine secretion in antigen presenting cells and vaccine-elicited neoantigen-specif

SUBMITTER: Luo Y 

PROVIDER: S-EPMC11880734 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature