Biomimetic integration of functionally controlled modular tissue building blocks for engineering 3D vascularized adipose tissue.

ABSTRACT: Adipose tissue is highly vascularized, which is crucial for homeostasis and energy storage. Current efforts to engineer 3D vascularized adipose tissue in vitro typically involve co-culturing adipocytes and endothelial cells, but adipogenic differentiation often suppresses endothelial function. In this study, we propose a novel approach to reconstruct vascularized adipose tissues in vitro by effectively coupling adipogenesis and vasculogenesis. First, we developed adipo-inductive nanofibers (ID/F@INS) that contain indomethacin and insulin. The in vitro adipogenesis of human adipose-derived stem cells (hADSCs) in general medium was significantly enhanced in adipogenic spheroids (AS) prepared with hADSCs and ID/F@INS, which were encapsulated in a gelatin methacryloyl (Gel

SUBMITTER: Lee S 

PROVIDER: S-EPMC11880769 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature