Mechanistic and structural insights into the itaconate-producing <i>trans</i>-aconitate decarboxylase Tad1.

ABSTRACT: Itaconic acid belongs to the high-value precursors for the production of biomass-based industrial compounds. It originates from the tricarboxylic acid cycle, and depending on the organism, it is produced by different biosynthetic routes. The basidiomycete fungus Ustilago maydis synthesizes itaconic acid via isomerization of cis-aconitic acid to trans-aconitic acid, and subsequent decarboxylation catalyzed by the trans-aconitate decarboxylase Tad1, which belongs to the aspartase/fumarase superfamily. Since no other decarboxylase has been identified within this protein superfamily, Tad1 constitutes a novel type of decarboxylase. Here, we present high-resolution crystal structures of Tad1, which, together with mutational analysis and nuclear magnetic resonance spec

SUBMITTER: Zheng L 

PROVIDER: S-EPMC11880804 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature