Fast event-based electron counting for small-molecule structure determination by MicroED.

ABSTRACT: Electron counting helped realize the resolution revolution in single-particle cryoEM and is now accelerating the determination of MicroED structures. Its advantages are best demonstrated by new direct electron detectors capable of fast (kilohertz) event-based electron counting (EBEC). This strategy minimizes the inaccuracies introduced by coincidence loss (CL) and promises rapid determination of accurate structures. We used the Direct Electron Apollo camera to leverage EBEC technology for MicroED data collection. Given its ability to count single electrons, the Apollo collects high-quality MicroED data from organic small-molecule crystals illuminated with incident electron beam flux densities as low as 0.01-0.045 e^-/Ų/s. Under even the lowest flux density (0.01 e

SUBMITTER: Vlahakis N 

PROVIDER: S-EPMC11881165 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature