A Novel Method for Thyroarytenoid Myofiber Culture.

ABSTRACT:

Objectives/hypothesis

Myofiber culture has been employed to investigate muscle physiology in vitro and is well-established in the rodent hind limb. Thyroarytenoid (TA) myofiber culture has not been described, providing an opportunity to employ this method to investigate distinct TA myofiber functions. The purpose of this study was to assess the feasibility of a TA myofiber culture model.

Study design

In vitro.

Methods

TA muscles from five Sprague Dawley rats were independently isolated and digested for 90 min. A smooth-tip, wide-bored pipette dissociated TA myofibers from cartilage, and the fibers were distributed on collagen-coated dishes and incubated at 37°C, 5% CO₂ for 2 h. Myofiber specificity was determined via immunolabeling for desmin and myosin hea

SUBMITTER: Gartling G 

PROVIDER: S-EPMC11881223 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature