Purine metabolism in bone marrow microenvironment inhibits hematopoietic stem cell differentiation under microgravity.

ABSTRACT:

Background

Spaceflight and microgravity environments have been shown to cause significant health impairments, including bone loss, immune dysfunction, and hematopoietic disorders. Hematopoietic stem cells (HSCs), as progenitors of the hematopoietic system, are critical for the continuous renewal and regulation of immune cells. Therefore, elucidating the regulatory mechanisms governing HSC fate and differentiation in microgravity environments is of paramount importance.

Methods

In this study, hindlimb unloading (HU) was employed in mice to simulate microgravity conditions. After 28 days of HU, cells were isolated for analysis. Flow cytometry and colony-forming assays were utilized to assess changes in HSC proliferation and differentiation. Additionally, transcriptomic and unt

SUBMITTER: Liu X 

PROVIDER: S-EPMC11881365 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature