Risk assessment of an Aedes flavivirus and its effect on pathogenic flavivirus replication in mosquitoes.

ABSTRACT:

Background

Mosquitoes are efficient vectors of medically significant flaviviruses and serve as hosts for insect-specific flaviviruses (ISFs). Aedes flavivirus (AEFV) is a classical ISF. Given the increasing discovery of ISFs, it is urgent to evaluate the potential risk of ISFs to human health as well as their impact on the transmission of pathogenic flaviviruses.

Methods

We isolated a strain of AEFV from wild Aedes albopictus populations in Hainan Province, China, using iodixanol density-gradient ultracentrifugation. The infection of the AEFV Hainan strain in Aedes, Culex, and four mammalian cell lines was investigated using fluorescence in situ hybridization (FISH) assays, and relative and absolute quantitative polymerase chain reaction (qPCR). Whether AEFV alters the vecto

SUBMITTER: Fu Y 

PROVIDER: S-EPMC11881423 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature